League of Legends was originally created as a mod for Warcraft III: The Frozen Throne, called Defense of the Ancients (DotA).

The voice actress for the champion Jinx, Sarah Anne Williams, is also a professional singer who performed the song "Get Jinxed" for the character.

The champion Nidalee's name is a reference to the American singer-songwriter Stevie Nicks, whose nickname is "The White Witch."

The character design for the champion Ezreal was inspired by the anime/manga series "Fullmetal Alchemist."

The champion Yorick's ultimate ability, "Omen of Death," can actually bring back a dead ally for a brief period of time.

The champion Singed's name is a play on words, as it is an anagram of the word "designs."

The champion Riven's in-game sword was actually created by a real-life swordsmith, Peter Lyon.

The champion Kha'Zix's design was inspired by the creatures from the movie "Predator."

The champion Anivia was initially designed to be a support champion, but was eventually changed to a mage due to her high damage potential.

The champion Warwick's ultimate ability, "Infinite Duress," was originally named "Hunter's Mark" but was changed due to copyright concerns.

The champion Malphite's design was inspired by the rock formation known as Ayers Rock/Uluru in Australia.